Effectivity of Bacillus thuringiensis from Soil in Freshwater Swamps against Epilachna sp. Larvae
Keywords:Bacillus thuringiensis, bioinsectide , Epilachna sp. , mortality
Bacillus thuringiensis is an entomopathogenic bacterium isolated from the soil and has been widely used as an active ingredient in the manufacture of bioinsecticides. The target insects are very specific and depend on the type of protein content. Epilachna sp. are important insect pests because both larvae and adults as plant-eating pests. The research aimed was to investigate the effectivity of B. thuringiensis against the larvae of Epilachna sp.. B. thuringiensis- bio-insecticide was prepared using isolates originally from freshwater swamp soil of South Sumatra (SMR04). Epilachna sp larvae were mass-reared with Solanum torvum leaf feed in the laboratory. The design used was a completely randomized design, with 6 treatments and 5 replications. Treatments were spore concentration contained in the bioinsecticide solution included: 1 x 108, 1 x 107, 1 x 106, 1 x 105 spores/mL, commercial bio-insecticide and without treatment as a control. Each replication used 10 individual of the 2nd larvae. Statistical test results showed larval mortality in B. thuringiensis treatment was significantly different from commercial bio-insecticide treatment. The highest mortality of bio-insecticide treatment occurred at a concentration of 108 spores/ml (40.00 %) and the lowest was at a concentration of 105 spores/mL (18.01 %). The lowest LT50 value of bio-insecticide treatment was at a concentration of 108 spores/mL, namely 79.37 hours. The control of larvae included in the Coleoptera order was still not satisfactory yet, considering the presence of protein content in B. thuringiensis strain SMR04 which did not match the type of protein required.
How to Cite
Copyright (c) 2021 Jurnal Lahan Suboptimal : Journal of Suboptimal Lands
This work is licensed under a Creative Commons Attribution 4.0 International License.
Jurnal Lahan Suboptimal: Journal of Suboptimal Lands article's license is CC-BY-SA 4.0.Â This license lets others distribute, remix, tweak, and build upon author's work, even commercially, as long as they credit the original creation.
Authors who publish with this journal agree to the following terms:
- Authors retain copyright and grant the journal right of first publication with the work simultaneously licensed under aÂ Creative Commons Attribution License-ShareAlike (CC BY-SA 4.0) licenseÂ that allows others to share the work with an acknowledgement of the work's authorship and initial publication in this journal.
- Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal's published version of the work (e.g., post it to an institutional repository or publish it in a book), with an acknowledgement of its initial publication in this journal.
- Authors are permitted and encouraged to post their work online (e.g., in institutional repositories or on their website) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (SeeÂ The Effect of Open Access).