Effectivity of Bacillus thuringiensis from Soil in Freshwater Swamps against Epilachna sp. Larvae


  • Yulia Pujiastuti Plant Protection Program, Fakulty of Agriculture, Universitas Sriwijaya, South Sumatra, Indonesia
  • Erni Indriani Plant Protection Program, Fakulty of Agriculture, Universitas Sriwijaya, South Sumatra, Indonesia
  • A Muslim Plant Protection Program, Fakulty of Agriculture, Universitas Sriwijaya, South Sumatra, Indonesia
  • Chandra Irsan Plant Protection Program, Fakulty of Agriculture, Universitas Sriwijaya, South Sumatra, Indonesia
  • Arsi Arsi Plant Protection Program, Fakulty of Agriculture, Universitas Sriwijaya, South Sumatra, Indonesia




Bacillus thuringiensis, bioinsectide , Epilachna sp. , mortality


Bacillus thuringiensis is an entomopathogenic bacterium isolated from the soil and  has been widely used as an active ingredient in the manufacture of bioinsecticides. The target insects are very specific and depend on the type of protein content. Epilachna sp. are important insect pests because both larvae and adults as plant-eating pests. The research aimed was to investigate the effectivity  of  B. thuringiensis against the larvae of Epilachna sp.. B. thuringiensis- bio-insecticide was prepared using isolates originally from freshwater swamp soil of South Sumatra (SMR04). Epilachna sp larvae were mass-reared with Solanum torvum leaf feed in the laboratory. The design used was a completely randomized design, with 6 treatments and 5 replications. Treatments were spore concentration contained in the bioinsecticide solution included: 1 x 108, 1 x 107, 1 x 106, 1 x 105 spores/mL, commercial bio-insecticide and without treatment as a control. Each replication used 10 individual of the 2nd larvae. Statistical test results showed larval mortality in B. thuringiensis treatment was significantly different from commercial bio-insecticide treatment. The highest mortality of bio-insecticide treatment occurred at a concentration of 108 spores/ml (40.00 %) and the lowest was at a concentration of 105 spores/mL (18.01 %). The lowest LT50 value of bio-insecticide treatment was at a concentration of 108 spores/mL, namely 79.37 hours. The control of larvae included in the Coleoptera order was still not satisfactory yet, considering the presence of protein content in B. thuringiensis strain SMR04 which did not match the type of protein required.

Abstract viewed = 191 times


Ali A, Javed K, Javed H, Kassi AK, Aslam MR, Hussain K, Ahmad T. 2017. Screening of different brinjal (Solanum melongena L.) cultivars against hadda beetle (Epilachna vigintioctopunctata F.) in Pothwar region. Journal of Entomology and Zoology Studies. 5(1): 786-791.

Apriliyanto E, Setiawan BH. 2019. Intencity of pest attack against some variety of eggplants and its effect to yield. Agrotech Res. J. 3(1): 8-12. DOI: 10.20961/agrotechresj.v3i1.25254.

Brunner-Mendoza, María del Rocío Reyes-Montes C, Soumya M, Michael J Bidochka, Conchita T. 2018. A review on the genus Metarhizium as an entomopathogenic microbial biocontrol agent with emphasis on its use and utility in Mexico, Biocontrol Science and Technology. 29(1): 1-21 DOI: 10.1080/09583157.2018.1531111.

Chowański S, Milena K, Paweł M, Grzegorz R. 2014. Synthetic insecticides-is there an alternative? Review Pol. J. Environ. Stud. 23(2): 291-302.

Damalas, Christos A, Ilias G Eleftherohorinos. 2011. Pesticide exposure, safety issues, and risk assessment indicators. International Journal of Environmental Research and Public Health. 8: 1402-1419. DOI: 10.3390/ijerph8051402.

Hanifa D. 2013. Application of Safety and Healthy Work in Agricultural Sector. Agricultural Ministry Republic of Indonesia: Indonesia.

Jouzani GS, Elena V, Reza S. 2017. Bacillus thuringiensis: a successful insecticide with new environmental features and tidings Mini-Review. Applied Microbiology and Biotechnology. 101: 2691–2711.

Kroschel J, Mujica N, Okonya JS, Alyokhin A. 2020. Insect Pests Affecting Potatoes in Tropical, Subtropical, and Temperate Regions. The Potato Crop: Lima, Peru.

Kaleka AS, Kaur N, Gaganpreet KB. 2019. Larval Development And Molting. Book Chapter. DOI: 10.5772/Intechopen.85530.

Khaliq A, Javed M, M Sohail M, Sagheer M. 2014. Environmental effects on insects and their population dynamics. A Journal of Entomology and Zoology Studies. 2(2): 1-7.

Patil PB, Gaikwad SM. 2019. Diversity and association of ladybird beetles with the agricultural crops. Journal of Emerging Technologies and Innovative Research. 6(5): 457-459.

Pujiastuti Y, Rohwati, Suwandi, Probowati D, Suparman, Arsy. 2018a. Toxicity Bacillus thuringiensis-based bio insecticide enriched with golden snail meat flour against worker and soldier castes of Coptotermes curvignathus (Isoptera: Termitidae). J. of Advanced Agricultural Technologies. 5(1): 41-45.

Pujiastuti Y, S Masyitoh, S Dirgahayu, T Kadapo, Hadikusuma SS, Effendy. 2018b. The use of golden snail meal to enrich Bacillus thuringiensis culture media and its effect on the bacterial toxicity against Spodoptera litura. J. HPT Tropika. 18(1): 23-30.

Pujiastuti Y, Gunawan B, Arsi, Suparman, Sulistyani DP, Sandi S. 2020a. Bacillus thuringiensis propagated in bio-urine media as a biological control of termite Coptotermes curvignathus and armyworm Spodoptera litura. IOP Conf. Ser.: Earth Environ. Sci. 468 012009: Bogor, Indonesia.

Pujiastuti Y, Arsi A, Sandi S. 2020b. Characteristics of Bacillus thuringiensis isolates indigenous soil of South Sumatra (Indonesia) and their pathogenicity against oil palm pests Oryctes rhinoceros (Coleoptrea: Scarabaeidae). Biodiversitas. 21(4): 1287-1294.

Sansinenea E. 2012. Discovery and Description of Bacillus thuringiensis. In book: Bacillus thuringiensis Biotechnology. Editors: Estibaliz Sansinenea. Publisher: Springer. DOI: 10.1007/978-94-007-3021-2_1.

Smith GH, JM Roberts, Pope TW. 2018. Terpene based biopesticides as potential alternatives to synthetic insecticides for control of aphid pests on protected ornamentals. Crop Protection. 110: 125–130. DOI: 10.1016/j.cropro.2018.04.011.

Supriadi. 2013. Optimalization of application on pesticide in controlling plant pests and diseases. J. Litbang Pert. 32(1): 1-9.

Valicente FH, Edmar Souza Tuelher, Maria Isabella Santos Leite Leite, Corina Macedo Vieira. 2010. Production of Bacillus thuringiensis biopesticide using commercial lab medium and agricultural by-products as nutrient sources. Revista Brasileira de Milho e Sorgo. 9:1-10. DOI: 10.18512/1980-6477/rbms.v9n1p1-11.

Yuantari MGC, Widiarnako B, Sunoko HR. 2013. The level of farmer’s knowledge on pesticide application (Case study in Curut Village, Penawangan sub district, Grobogan District). In: Proceeding of national seminar on natural resorces and environmnet management. Indonesia. p. 1-8.




How to Cite

Pujiastuti, Y. ., Indriani, E. ., Muslim, A., Irsan, C. ., & Arsi, A. . (2021). Effectivity of Bacillus thuringiensis from Soil in Freshwater Swamps against Epilachna sp. Larvae . Jurnal Lahan Suboptimal : Journal of Suboptimal Lands, 10(1), 46–53. https://doi.org/10.36706/JLSO.10.1.2021.531




Most read articles by the same author(s)