Application of omega 3,6 with Moringa oleifera suplemented vitamin E on rumen fluid characteristics, microbial protein synthesis, and methane gas production in goats on suboptimal lands

Authors

  • Angelia Utari Department of Animal Husbandry, Faculty of Agriculture, Graha Nusantara University. Campus Tor I Simarsayang, Padangsidimpuan City 22712, North Sumatra, Indonesia
  • Rikardo Silaban Department of Livestock Production Technology, Politeknik Negeri Lampung. Rajabasa Raya, Kota Bandar Lampung 35141, Lampung, Indonesia
  • Novita Aswan Department of Agribusiness, Faculty of Agriculture, Graha Nusantara University. Campus I Tor Simarsayang, Padangsidimpuan 22712, North Sumatera, Indonesia
  • Toga Mahaji Department of Social Economy Fisheries, STPK Matauli. Pandan City, Central Tapanuli 22538, North Sumatera, Indonesia

DOI:

https://doi.org/10.36706/jlso.13.2.2024.670

Keywords:

goat, methan gas, moringa leaves, omega 3&6, vitamin E

Abstract

Changes in feeding patterns were needed to overcome the problem of methane gas emissions from ruminant livestock and the problem of low productivity at the smallholder livestock level. This solution involves direct action against the gas produced in the stomach of ruminants by providing feed containing active substances to reduce the microbes that produce gas in the stomach. One alternative feed that was often used as a promoter of improving rumen fluid characteristics was the use of omega 3.6 sources together with Moringa leaves enriched with vitamin E. This research aimed to investigate the effect of feeding on methane gas production in goats. The research method used a Randomized Block Design (RAK) with four treatments and five replications in vitro. From the research results, it could be concluded that the use of omega 3.6 together with Moringa leaves enriched with vitamin E was able to reduce methane gas production by 25.62% with a Volatile Fatty Acid (VFA) value of 144.53 mM, pH around 6.99, NH3 11.87 mg/100ml, microbial biomass 20.42 mg/ml, microbial protein 217.58mg/ml, bacterial colony 5.34x109 cells/ml and protozoa population 29x106 cells/ml. This conclusion shows that the use of omega 3.6 with Moringa oleifera supplemented with vitamin E has the potential to consistently improve rumen fluid characteristics and reduce methane gas emissions by using a combination of omega 3.6 sources and Moringa leaves in suboptimal land, as well as vitamin E supplementation.

References

Abuelfatah, K., Zuki, A. B., Goh, Y. M., & Sazili, A. Q. (2016). Effects of feeding whole linseed on ruminal fatty acid composition and microbial population in goats. Animal Nutrition, 2(4), 323–328. https://doi.org/10.1016/j.aninu.2016.10.004

Angelia, U., & Silaban, R. (2019). Phenolic content and cytotoxicity of extracts, antimicrobial compounds, and andaliman fruit (zanthoxylumacanthopodium) essential oil. Jurnal Graha Tani. Universitas Graha Nusantara Padangsidimpuan, 5(3), 22–27.

Agung, A., Oka, G., Made, L., Pendidikan, M., Dokter, P., Hewan, F. K., & Udayana, U. (2016). Identification of chemical compounds in ethanol extract of moringa leaves (Moringa oleifera L) in Bali. Indonesia Medicus Veterinus, 5(5), 464–473.

Bhatta, R., Saravanan, M.M., Baruah, L., & Prasad, C. S. (2015). Effect of graded level of tannin-containing tropical tree leaves on in vitro rumen fermentation, total protozoa and methane production. Journal Application Microbiology, 118, 557–564.

Bunglavan, S. J., & N. Dutta. (2013). Use of tannins as organic protectants of proteins in digestion of ruminants. Journal Livestock Science, 4, 67–77.

Danang, M., & Yulianto, E. (2016). Performance of bligon goats after the introduction of superior studs in the Purwo Manunggal Livestock Group, Gunungkidul. Jurnal Sain Veteriner, 34(2), 251–258. https://doi.org/10.22146/jsv.27568

Hindratiningrum, N., M. Bata dan S. A. Santosa. (2011). Produk fermentasi rumen dan produksi protein mikroba sapi lokal yang diberi pakan jerami amoniasi dan beberapa bahan pakan sumber energy. Agripet. 11 (2): 29–34.

Jayanegara, Anuraga, Goel, G., Makkar, H. P. S., & Becker, K. (2015). Methane emission, rumen fermentation and microbial population in vitro. Animal Feed Science and Technology. https://doi.org/10.1016/j.anifeedsci.2015.08.002

Laka, M.R., & Kleden, M.M. (2019). The effect of feeding completely fermented gamal litter and banana stems with different balances on the blood biochemistry of peanut goats. Jurnal Peternakan Lahan Kering, 1(4), 570–578. https://doi.org/10.57089/jplk.v1i4.462

Harahap, A. U., Warly, L., Hermon, Suyitman, & Evitayani. (2021). Testing the phytochemical content of jackfruit leaves (Artocarpus heteropyllus) and moringa leaves (Moringa oleifera) as additional feed for goats. Pastura, 10(1), 1-4. https://doi.org/10.24843/pastura.2021.v10.i02.p01

Morgavi, D. P., Forano, E., Martin, C., & Newbold, C. J. (2010). Microbial ecosystem and methanogenesis in ruminants. Animal, 4(7), 1024–1036. https://doi.org/10.1017/S1751731110000546

Mosoni, P., Martin, C., Forano, E., & Morgavi, D. P. (2011). Long-term defaunation increases the abundance of cellulolytic ruminococci and methanogens but does not affect the bacterial and methanogen diversity in the rumen of sheep. Journal of Animal Science, 89(3), 783–791. https://doi.org/10.2527/jas.2010-2947

Newbold, C. J., & Ramos-Morales, E. (2020). Review: Ruminal microbiome and microbial metabolome: Effects of diet and ruminant host. Animal. 14(S1): S78–S86. https://doi.org/10.1017/S1751731119003252

Rahmawati, P.D., Pangestu, E., Nuswatara, L. K., & Christiyanto, M. (2021). Digestibility of dry matter, organic matter, crude fat and total digestible nutrient value of goat forage. Jurnal Agripet, 21(1), 71–77. https://doi.org/10.17969/agripet.v21i1.17933

Suyadi, F. P., & Wahyuningsih, S. (2017). Reproductive performance in crosses of boer goats and etawah crossbreeds (PE). Jurnal Ilmu-Ilmu Peternakan, 23(1), 11–17.

Suyitman, Warly, L., Hellyward, J., & Pazla, R. (2021). Optimization of rumen bioprocess through the addition of phosphorus and sulfur minerals on ammoniated palm leaves and fronds (Elaeis guineensis jacq.). American Journal of Animal and Veterinary Sciences. https://doi.org/10.3844/ajavsp.2021.225.232

Tandi, J., Ikbal, M., & Andi A.M. (2018). Test of ethanol extract of jackfruit leaves on the histopathological features of the pancreas of male white rats induced by streptozotocin. Farmakologika Jurnal Farmasi, 15(2), 106–113.

Tavendale, M.H. L.P. Meahger, D. Pacheco, N. Walker, G.G. Attwood & S. Sivakumaran. (2005). Methane production from in vitro rumen incubation with Lotus pondoculatus and Medicago sativa and effects of extracable condensed tannin fraction on methanogenesis. Anim. Feed Sci. and Technol. 123-124, 403- 419.

Uddin, M. J., Khandaker, Z. H., Khan, M. J., & Khan, M. M. H. (2015). Dynamics of microbial protein synthesis in the rumen - A Review. Annals of Veterinary and Animal Science, 2(5), 116–131.

Verma, & Shukla, N. (2015). Impact of various factors responsible for fluctuations in plant secondary metabolite. J. Appl. Res. Med. Aromat. Plants, 2(4), 105–113.

Wahyono, T., Sasongko, W. T., Sholihah, M., and Pikoli, M. R. (2017). Effect of Addition of Jackfruit (Artocarpus heterophyllus) Leaf Tannin on the Biological Value of Moringa Leaves (Moringa oleifera) and Mung Bean Straw (Vigna radiata) In vitro. Livestock Bulletin, 41(1): 15–25. https://doi.org/10.21059/buletinpeternak.v41i1.22450

Wang, B., Jia, M., Fang, L., Jiang, L., & Li, Y. (2018). Effects of eucalyptus oil and anise oil supplementation on rumen fermentation characteristics, methane Emission, and digestibility in sheep. Journal of Animal Science, 96(8), 3460–3470. https://doi.org/10.1093/jas/sky216

Warly, L., Suyitman, Evitayani, & Fariani, A. (2015). Supplementation of solid Ex-decanter on performance of cattle fed palm fruit by-products. Pakistan Journal of Nutrition. https://doi.org/10.3923/pjn.2015.818.821

Wood, R. A., Vellinga, M., Thorpe, R., Trans, P., & Lond, R. S. (2018). Global warming and thermohaline circulation stability global warming and thermohaline circulation stability. August. https://doi.org/10.1098/rsta.2003.1245

Zain, M., Jurnida, R., Khasrad, & Erpomen. (2015). In vitro fermentation characteristics of palm oil by products which is supplemented with growth factor rumen microbes. Pakistan Journal of Nutrition, 14 (9), 625–628.

Downloads

Published

2024-10-01

How to Cite

Utari, A., Silaban, R., Aswan, N., & Mahaji, T. (2024). Application of omega 3,6 with Moringa oleifera suplemented vitamin E on rumen fluid characteristics, microbial protein synthesis, and methane gas production in goats on suboptimal lands. Jurnal Lahan Suboptimal : Journal of Suboptimal Lands, 13(2), 166–173. https://doi.org/10.36706/jlso.13.2.2024.670

Issue

Section

Articles

Most read articles by the same author(s)