The Potential of Nutmeg’s Microbes (Myristica fragrans Houtt.) as Antagonistic Agents against Rigidoporus microporus

Authors

  • Dwi N Susilowati Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Bogor 16111, West Java, Indonesia
  • Sri Rahayuningsih Indonesian Center for Estate Crops Research and Development, Bogor 16111, West Java, Indonesia
  • Indah Sofiana Department of Biology, Faculty of Mathematics and Natural Sciences, Jakarta State University, Campus A, Jakarta Timur 13220, Indonesia
  • Nani Radiastuti Faculty of Science and Technology, UIN Syarif Hidayatullah Jakarta, Ciputat, Tangerang Selatan 15412, Banten, Indonesia

DOI:

https://doi.org/10.36706/JLSO.10.1.2021.529

Keywords:

bacillus aerius , bacillus subtillis , chitinase enzymes , hemolysis

Abstract

This study aimed to obtain yeast and bacteria from Myristica fragrans Houtt., which have the potential to produce chitinase enzymes with antagonistic ability against Rigidoporus microporous. Both microorganisms were extracted from the leaves and fruit of nutmeg. A total of 35 yeast and 29 bacterial isolates were obtained, with different morphological characters. The chitinolytic test was carried out qualitatively, and the parameters observed include the clear zones around the colony. A total of 4 bacterial isolates produced chitinase enzymes (BP 1.2.1, BP 2.1.1, EPBj II.K1, and EPBj II. K2) with a chitinolytic index of 3.92, 5.38, 2.00, and 1.66, respectively. Yeast isolates were negative for chitinase enzymes. The difference in index value indicated a variation in enzyme activity. The antagonist test was carried out using a dual culture method. A total of 1 yeast and 14 bacterial isolates inhibited the growth of R. microporous, and each has a different inhibitory zone. Based on the percentage of inhibition value, the highest percentage occurred in P.K1(41.1%), P. K2 (50%), dan EPBj II. K6 (42.2%). The antagonist test indicator includes the formation of inhibitory zones on the medium. Hemolysis test showed that yeast and bacteria are not able to break down blood cells in the medium. The molecular identification showed that P. K1 and P. K2 isolates were classified as Bacillus subtillis and EPBj II. K6 were identified as Bacillus aerius with 100% sequence homology and 99% bootstrap value respectively. These findings provided information about potential microbes that control white root fungus.       


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References

Agbogidi OM, Azagbaekwe OP. 2013. Health and nutritional benefits of nutmeg

(Mystica fragrans houtt.). Sci. Agri. 1(2): 40-44.

Adam SIA. 2016. Isolation and Identification of Streptococci Transmitted by House Fly Musca domestica Collected from Fly Breeding Sites in Wad Medani, Gezira State, Sudan (2015-2016). [Ph.D. thesis]. University of Gezira.

Andreote FD, eSilva MDCP. 2017. Microbial communities associated with plants: learning from nature to apply it in agriculture. Current Opinion in Microbiology. 37: 29-34.

Antwis RE, Sarah M. Griffiths, Xavier A. Harrison, Paz Aranega-Bou, Andres Arce. 2017. Fifty important research questions in microbial ecology. FEMS Microbiology Ecology. 93(5): 1-10. DOI: 10.1093/femsec/fix044.

Beier S, Bertilsson S. 2013. Bacterial chitin degradation-mechanisms and ecophysiological strategies. Frontiers in Microbiology. 4:149.

Bermawie N, Ma'mun NFN, Purwiyanti S. 2018. Selection of nutmeg mother trees in the germplasm collection at Cicurug experimental station Sukabumi. Bulletin of Research on Spice and Medicinal Crops. 29(1): 21-36.

Campbell NA, Reece JB, Mitchell LG. 2012. Biologi Edisi ke-5 Jilid 1. Terjemahan: Lestari R. Erlangga: Jakarta.

Campbell BJ, Kirchman DL. 2013. Bacterial diversity, community structure and potential growth rates along an estuarine salinity gradient. The ISME Journal. 7(1): 210-220.

Chen PH, Chen RY, Chou JY. 2018. Screening and evaluation of yeast antagonists for biological control of Botrytis cinerea on strawberry fruits. Mycobiology. 46(1): 33-46.

Cihan AC, Tekin N, Ozcan B, Cokmus C. 2012. The genetic diversity of genus Bacillus and the related genera revealed by 16S rRNA gene sequences and ardra analyses isolated from geothermal regions of Turkey. Brazilian Journal of Microbiology. 43(1): 309-324.

Crawford JW, Deacon, L, Grinev, D., Harris, JA, Ritz K, Singh BK, Young I. 2012. Microbial diversity affects self-organization of the soil–microbe system with consequences for function. Journal of the Royal Society Interface. 9(71): 1302-1310.

Bharadwaj L, Bhardwaj M, Sharma MK. 2012. An analysis of Fe2O3 assisted photocatalytic degradation of Congo Red dye. Toxicol. Environ. Health Sci. 4: 62–69. DOI: 10.1007/s13530-011-0102-5.

Faye A, Pintaud JC, Baker WJ, Vigouroux Y, Sonke B, Couvreur TL. 2016. Phylogenetics and diversification history of African rattans (Calamoideae, Ancistrophyllinae). Botanical Journal of the Linnean Society. 182(2): 256-271.

Ghosh S, Mohanty S, Nayak S, Sukla L B, Das AP. 2016. Molecular identification of indigenous manganese solubilising bacterial biodiversity from manganese mining deposits. Journal of Basic Microbiology. 56(3): 254-262.

Goh YK, Marzuki NF, Liew YA, Goh KJ. 2018. Antagonistic effects of fungicolous ascomycetous Cladobotryum semicirculare on Rigidoporus microporus white root disease in rubber trees (Hevea brasiliensis) under in vitro and nursery experiments. Journal of Rubber Research. 21(1): 62-72.

Haliza W, Suhartono MT. 2012. Karakteristik kitinase dari mikrobia. Bul. Teknol. Pascapanen Pert. 8(1): 1-14.

Jamsari J, Kamelia R, Syukur S, Syukriani, L, Ferita I. 2018. AJAB. Asian J Agri & Biol. 6(1): 95-102.

Landum MC, Do Rosario Felix M, Alho J, Garcia R, Cabrita MJ, Rei F, Varanda CM. 2016. Antagonistic activity of fungi of Olea europaea L against Colletotrichum acutatum. Microbiological Research. 183: 100-108.

Louca S, Mazel F, Doebeli M, Parfrey LW. 2019. A census-based estimate of earth's bacterial and archaeal diversity. PLoS Biology. 17(2): e3000106.

Madigan MT, Martinko JM, Stahl DA, Clark DP. 2012. Brock Biology of Microorganisms. 13th ed. Boston: Pearson education, Inc.

Moulia MN, SetyabudiS, Salleh B, Rahayu ES. 2014. Penicillium species isolated from cocoa, coffee beans, and dried cassava in Yogyakarta Indonesia and their ochratoxin production. Indonesian Food and Nutrition Progress. 13(1): 1-10.

Moyano SR, Martín A, Villalobos MC, Calle A, Serradilla MJ, Córdoba MG, Hernández A. 2016. Yeasts isolated from figs (Ficus carica L.) as biocontrol agents of postharvest fruit diseases. Food Microbiol. 57: 45-53.

Nega A. 2014. Review on concepts in biological control of plant pathogens. Journal of Biology, Agriculture and Healthcare. 4(27): 33-54.

Nunes CA. 2012. Biological control of postharvest diseases of fruit. Eur. J. Plant Pathol. 133: 181-96.

Nurhasanah. 2014. Antimicrobial activity of nutmeg (Myristica fragrans Houtt) fruit methanol extract againts growth Staphylococus aureus and Escherichia coli. Jurnal Bio Edukasi. 3(1): 277–286.

Okukpe KM, Adeloye MA, Alli OI, Adeyina OA, Annongu, A. 2012. Investigation of pytohormonal potential of some selected tropical plants. Res J. Med Plants. 6(6): 425-432.

Rahardiyan D, Poluakan M, Moko EM. 2020. Physico-chemical properties of nutmeg (Myristica fragrans houtt) of North Sulawesi nutmeg. Fullerene Journal of Chemistry. 5(1): 23-31.

Scheuerl T, Hopkins M, Nowell RW, Rivett DW, Barraclough TG, Bell T. 2020. Bacterial adaptation is constrained in complex communities. Nature Communications. 11(1): 1-8.

Srinivasan R, Karaoz U, Volegova M, MacKichan J, Kato-Maeda M, Miller S, Lynch SV. 2015. Use of 16S rRNA gene for identification of a broad range of clinically relevant bacterial pathogens. PloS one. 10(2): e0117617.

Susilowati DN, Haryono U. 2018. Growth inhibition of Aspergillus spp. by endophytic bacteria. Jurnal Agro Biogen. 14(1):47.

Sofiana I, Susilowati DN, Putra IP. 2020. The potential of endophytic fungi as biocontrol and phospate solubilization agent in capsicum anuum. Fungal Territory. 3(3): 16-19.

Talibi I, Boubaker H, Boudyach EH, Ait Ben Aoumar A. 2014. Alternative methods for the control of postharvest citrus diseases. Journal of Applied Microbiology. 117(1): 1-17.

Tan KP, Khoo HE, Azrina A. 2013. Comparison of antioxidant components and antioxidant capacity in different parts of nutmeg (Myristica fragrans). International Food Research Journal. 20(3): 1049.

Turista DDR, Puspitasari E. 2019. The growth of Staphylococcus aureus in the blood agar plate media of sheep blood and human blood groups A, B, AB, and O. Jurnal Teknologi Laboratorium. 8(1): 1-7.

Yadav IC, Devi NL. 2017. Pesticides classification and its impact on human and environment. Environmental science and Engineering. 6: 140-158.

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Published

2021-04-01

How to Cite

Susilowati, D. N., Rahayuningsih, S. ., Sofiana, I. ., & Radiastuti, N. . (2021). The Potential of Nutmeg’s Microbes (Myristica fragrans Houtt.) as Antagonistic Agents against Rigidoporus microporus. Jurnal Lahan Suboptimal : Journal of Suboptimal Lands, 10(1), 1–13. https://doi.org/10.36706/JLSO.10.1.2021.529

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