TY - JOUR AU - Pujiastuti, Yulia AU - Indriani, Erni AU - Muslim, A AU - Irsan, Chandra AU - Arsi, Arsi PY - 2021/04/05 Y2 - 2024/03/29 TI - Effectivity of Bacillus thuringiensis from Soil in Freshwater Swamps against Epilachna sp. Larvae JF - Jurnal Lahan Suboptimal : Journal of Suboptimal Lands JA - J. Lahan Suboptimal VL - 10 IS - 1 SE - Articles DO - 10.36706/JLSO.10.1.2021.531 UR - https://jlsuboptimal.unsri.ac.id/index.php/jlso/article/view/531 SP - 46-53 AB - <p><em>Bacillus thuringiensis</em> is an entomopathogenic bacterium isolated from the soil and  has been widely used as an active ingredient in the manufacture of bioinsecticides. The target insects are very specific and depend on the type of protein content. <em>Epilachna</em> sp. are important insect pests because both larvae and adults as plant-eating pests. The research aimed was to investigate the effectivity  of  <em>B. thuringiensis</em> against the larvae of <em>Epilachna</em> sp.. <em>B. thuringiensis</em>- bio-insecticide was prepared using isolates originally from freshwater swamp soil of South Sumatra (SMR04). <em>Epilachna</em> sp larvae were mass-reared with <em>Solanum torvum</em> leaf feed in the laboratory. The design used was a completely randomized design, with 6 treatments and 5 replications. Treatments were spore concentration contained in the bioinsecticide solution included: 1 x 10<sup>8</sup>, 1 x 10<sup>7</sup>, 1 x 10<sup>6</sup>, 1 x 10<sup>5</sup> spores/mL, commercial bio-insecticide and without treatment as a control. Each replication used 10 individual of the 2nd larvae. Statistical test results showed larval mortality in <em>B. thuringiensis</em> treatment was significantly different from commercial bio-insecticide treatment. The highest mortality of bio-insecticide treatment occurred at a concentration of 10<sup>8 </sup>spores/ml (40.00 %) and the lowest was at a concentration of 10<sup>5</sup> spores/mL (18.01 %). The lowest LT<sub>50</sub> value of bio-insecticide treatment was at a concentration of 10<sup>8</sup> spores/mL, namely 79.37 hours. The control of larvae included in the Coleoptera order was still not satisfactory yet, considering the presence of protein content in <em>B. thuringiensis</em> strain SMR04 which did not match the type of protein required.</p> ER -